Gram stain is used to differentiate bacterial species microscopically. With this stain, bacteria are divided into two main groups as Gram Positive (+), Purple stained and Gram Negative (-), Pink stained. If it is desired to examine cells such as blood, epithelium, cells from the samples by staining with Gram stain, it is recommended to fix the smears for one minute with methanol before staining. In addition, the morphology of bacteria such as cocci, rods, clusters, and chain formation are defined.
Gram stain is used to differentiate bacterial species microscopically. With this stain, bacteria are divided into two main groups as Gram Positive (+), Purple stained and Gram Negative (-), Pink stained. Yeast cells such as Candida can also be stained with Gram stain and are observed as Gram positive. Lugol solution with PVP used after crystal violet in dyeing provides better chemical bonding of crystal violet to the cell wall. Bacteria staining purple in Gram-stained smears are defined as Gram-positive, and bacteria staining pink are defined as Gram-negative.
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