Fluorochrome staining method using Auramine-Rhodamine has an important place in the diagnosis of tuberculosis. It is used in the staining of smears prepared to detect the presence of mycobacteria in clinical samples or culture media. In microscopic examination, fluorescent stained mycobacteria are detected more easily than bacteria stained with Carbol Fuchsin, making scanning easier and shorter. Ehrlich Ziehl Neelsen (EZN) or Kinyoun staining on smears examined with fluorescent dye It should be noted that more false positive results can be obtained than other methods. Auramine-Rhodamine solution is a fluorescent dye solution containing Phenol. Mycobacteria give bright yellow green fluorescence in smears stained with Auramine-Rhodamine dye. Other microorganisms and cell debris are not seen in the examination as they are stained black with potassium permanganate.
CATALOG
